Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Neural

Cell type

Hippocampus

MeSH Description

A curved elevation of GRAY MATTER extending the entire length of the floor of the TEMPORAL HORN of the LATERAL VENTRICLE (see also TEMPORAL LOBE). The hippocampus proper, subiculum, and DENTATE GYRUS constitute the hippocampal formation. Sometimes authors include the ENTORHINAL CORTEX in the hippocampal formation.

Sample

source_name

whole hippocampus

age

P30

tissue

brain

tissue compartment

hippocampus

strain

C57BL/6N

genotype

Kansl1 wt control

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chipping for H3k4me3 (Diagenode A5051-001P) was performed as in http://www.blueprint-epigenome.eu/UserFiles/file/Protocols/Histone_ChIP_July2014.pdf Library was synthetized with KAPA Hyper prep kit (KK8504) according to manufacturer’s instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

54325352

Reads aligned (%)

95.3

Duplicates removed (%)

38.6

Number of peaks

27421 (qval < 1E-05)

mm9

Number of total reads

54325352

Reads aligned (%)

95.2

Duplicates removed (%)

38.6

Number of peaks

27426 (qval < 1E-05)