Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K9me3

Cell type

Cell type Class
Others
Cell type
Olfactory epithelium
NA
NA

Attributes by original data submitter

Sample

source_name
Olfactory Epithelium
strain/background
Mixed
tissue
Regenerated Olfactory Epithelium
treatment
50 mg/kg Methimazole Group 4
genotype
Lmnb1-/-
antibody
H3K9me3 (1 ug/ml, Abcam ab8898)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Approximately 500,000 Tomato-positive mutant or heterozygous olfactory epithelial cells were sorted from a single mouse. Each sample was crosslinked, sonicated and subjected to ChIP with either anti-H3K9me3 or anti-H3K4me3. Libraries were prepared from precipitated DNA and sequenced, producing approximately 50 million 50 bp reads per sample. Libraries were prepared using Illumina Prep Kit

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
50714516
Reads aligned (%)
90.2
Duplicates removed (%)
38.6
Number of peaks
689 (qval < 1E-05)

mm9

Number of total reads
50714516
Reads aligned (%)
89.7
Duplicates removed (%)
38.5
Number of peaks
770 (qval < 1E-05)

Base call quality data from DBCLS SRA