Antigen

Antigen Class

TFs and others

Antigen

BRD4

Cell type

Cell type Class

Blood

Cell type

MOLM-13

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

MOLM-13

cell line

MOLM-13

cell type

acute myeloid leukemia

chip antibody

BRD4 (Bethyl Laboratories, # A301-985A100)

Sequenced DNA Library

library_name

GSM6448431

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Fixed numbers of mouse or drosophila cells were spiked-in to treated cells. Cells were then lysed. Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries were prepared with Takara ThruPLEX® DNA-Seq Kit (Cat. No. R400674) according to manufacturer's instructions.

Sequencing Platform

instrument_model

NextSeq 2000

hg38

Number of total reads

44633454

Reads aligned (%)

81.8

Duplicates removed (%)

28.3

Number of peaks

15940 (qval < 1E-05)

hg19

Number of total reads

44633454

Reads aligned (%)

81.1

Duplicates removed (%)

29.7

Number of peaks

15672 (qval < 1E-05)