Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYCN

Cell type

Cell type Class
Neural
Cell type
KELLY
Primary Tissue
Brain
Tissue Diagnosis
Neuroblastoma

Attributes by original data submitter

Sample

source_name
KELLY_Neuroblastoma
cell line
KELLY
cell type
neuroblastoma cell line
growth condition
10% FBS, DMEM
treated with
None
chip antibody
MYCN
chip antibody vendor
abcam
input
KELLY_INPUT
barcode
TTCCATTG

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using the Rubicon Thruplex FD library preparation kit (cat#: 400427) per manufacturers instructions.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
60987732
Reads aligned (%)
87.4
Duplicates removed (%)
10.6
Number of peaks
10730 (qval < 1E-05)

hg38

Number of total reads
60987732
Reads aligned (%)
88.9
Duplicates removed (%)
9.6
Number of peaks
10947 (qval < 1E-05)

Base call quality data from DBCLS SRA