Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

mESC

strain

129/Ola

antibody

h3k4me1

antibody vendor

abcam

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Crosslinkedand sonicated chromatin was incubated with the ab indicated. Complex associated DNA was purified and sequenced. Libraries were constructed as per manufacturers instructions for Illumina Tru-seq.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

45241184

Reads aligned (%)

89.6

Duplicates removed (%)

8.2

Number of peaks

629 (qval < 1E-05)

mm9

Number of total reads

45241184

Reads aligned (%)

89.3

Duplicates removed (%)

8.2

Number of peaks

772 (qval < 1E-05)