Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

mESC

strain

129/C57

antibody

h3k27ac

genotype

MLL3/4 flox flox parental

antibody vendor

active motif

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Crosslinkedand sonicated chromatin was incubated with the ab indicated. Complex associated DNA was purified and sequenced. Libraries were constructed as per manufacturers instructions for Illumina Tru-seq.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

25559713

Reads aligned (%)

90.8

Duplicates removed (%)

16.8

Number of peaks

3722 (qval < 1E-05)

mm9

Number of total reads

25559713

Reads aligned (%)

90.5

Duplicates removed (%)

16.9

Number of peaks

3688 (qval < 1E-05)