GSM2108793: Input Tbx1 HET +TCP rep1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Embryo
Cell type
E9.5 embryos
NA
NA
Attributes by original data submitter
Sample
source_name
Mouse embryos
cell type
E9.5
treatment
TCP
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP analysis, P19Cl6 cells and mouse embryos were cross-linked in 1% formaldehyde and chromatin was extracted and sonicated in microtubes using the Covaris® S2 instrument. Soluble chromatin was incubated with TBX1 antibody, H3K4me1 and H3K27Ac antibodies. For expression analysis, RNA was extracted from E9.5 embyos and P19Cl6 cells at day 1 during differentiation. For ChIP analysis, ChIP DNA or input samples were prepared for Solexa and Solid sequencing using Chip-Seq Sample Preparation kit and TruSeq SBS kit v3 HS or NEB Next DNA Simple Prep Master Mix set 3. For RNA-seq libraries preparation we used the Illumina's strand specific RNA seq protocol.