GSM2108113: MP41 BAP1 positive rep 2 H3K27me3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27me3
Cell type
Cell type Class
Others
Cell type
Uveal melanoma
NA
NA
Attributes by original data submitter
Sample
source_name
Patient derived Xenograft derived from Primary Tumor of Uveal Melanoma
tissue
Tumor of Uveal Melanoma
chip antibody
H3K27me3
bap1 status
positive
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed from 6 millions of cells originating from Patient Derived Tumour grafts; magnetic Dynabeads coupled to Protein A were used for the IP (Invitrogen). Sonication was performed to obtain fragment size of 150-300 bp. Libraries were prepared accordingly to manufactures (TruSeq ChIP sample Prep Kit, Illumina). Sequencing was performed on a Illumina Hi-Seq 2500.