Antigen

Antigen Class

TFs and others

Antigen

PAX8

Cell type

Cell type Class

Gonad

Cell type

OVSAHO

Primary Tissue

Ovary

Tissue Diagnosis

Adenocarcinoma Papillary Serous

Sample

source_name

OVSAHO

cell line

OVSAHO

cell line type

High grade serous ovarian carcinoma

antibody

PAX8 antibody (Novus, Product #NBP1-32440, Littleton, CO)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with PAX8 antibody (Novus, Product #NBP1-32440, Littleton, CO). After repairing the DNA ends, sequencing adaptors (Illumina, San Diego, CA) were ligated to the purified chromatin, and the DNA was amplified for sequencing

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

1402124

Reads aligned (%)

47.9

Duplicates removed (%)

7.4

Number of peaks

374 (qval < 1E-05)

hg19

Number of total reads

1402124

Reads aligned (%)

36.8

Duplicates removed (%)

25.2

Number of peaks

517 (qval < 1E-05)