Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
PAX8

Cell type

Cell type Class
Uterus
Cell type
FT33
NA
NA

Attributes by original data submitter

Sample

source_name
FT33
cell line
FT33
cell line type
Immortalized fallopian tube secretory cell
antibody
PAX8 antibody (Novus, Product #NBP1-32440, Littleton, CO)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with PAX8 antibody (Novus, Product #NBP1-32440, Littleton, CO). After repairing the DNA ends, sequencing adaptors (Illumina, San Diego, CA) were ligated to the purified chromatin, and the DNA was amplified for sequencing

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
773065
Reads aligned (%)
43.4
Duplicates removed (%)
22.9
Number of peaks
509 (qval < 1E-05)

hg38

Number of total reads
773065
Reads aligned (%)
68.0
Duplicates removed (%)
8.2
Number of peaks
345 (qval < 1E-05)

Base call quality data from DBCLS SRA