Antigen

Antigen Class

TFs and others

Antigen

NEUROD1

Cell type

Cell type Class

Lung

Cell type

NCI-H446

Primary Tissue

Lung

Tissue Diagnosis

Carcinoma Small Cell

Sample

source_name

H446

cell line

H446

cell type

small cell lung cancer SCLC-N subtype

genotype

WT

chip antibody

NEUROD1 (CST, #4373)

Sequenced DNA Library

library_name

GSM6421554

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a Sonic Dismembrator (Fisher brand Model 705). Libraries were constructed using entire ChIPed DNA or 300 ng of the input DNA using a NEBNext Ultra II DNA Library Prep kit with AMPure XP magnetic beads (Beckman Coulter). Estimation of library quality and quantity was performed using a Bioanalyzer and Qubit assays.

Sequencing Platform

instrument_model

Illumina HiSeq 3000

hg38

Number of total reads

27863239

Reads aligned (%)

98.6

Duplicates removed (%)

11.6

Number of peaks

55202 (qval < 1E-05)

hg19

Number of total reads

27863239

Reads aligned (%)

98.2

Duplicates removed (%)

12.5

Number of peaks

55170 (qval < 1E-05)