Antigen

Antigen Class

Histone

Antigen

H3K4me2

Cell type

Cell type Class

Embryo

Cell type

Embryonic microglia

NA

NA

Sample

source_name

E12.5 microglia k4me2

strain

CX3CR1-GFP+ C57BL/6J

tissue

Brain

cell type

E12.5 microglia

chip antibody

K4me2 (Abcam, #ab32356)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Lara-Astiaso D, Weiner A, Lorenzo-Vivas E, Zaretsky I et al. Immunogenetics. Chromatin state dynamics during blood formation. Science 2014 Aug 22;345(6199):943-9. PMID: 25103404 indexing-first chromatin immunoprecipitation and ATAC-seq

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

23724839

Reads aligned (%)

97.9

Duplicates removed (%)

28.5

Number of peaks

5639 (qval < 1E-05)

mm9

Number of total reads

23724839

Reads aligned (%)

97.8

Duplicates removed (%)

29.5

Number of peaks

5594 (qval < 1E-05)