Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Pancreas

Cell type

MIN6B1

NA

NA

Sample

source_name

MIN6 cells

cell type

Insulinoma derive pancreatic beta cells

chip antibody

Nkx2.2 (Sigma-Hpa003468)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and Nkx2.2-DNA complexes were isolated with Nkx2.2 antibody. Libraries were prepared as per instructions of the Kapa Hyper kit (Illumina)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

25704681

Reads aligned (%)

93.9

Duplicates removed (%)

10.1

Number of peaks

488 (qval < 1E-05)

mm9

Number of total reads

25704681

Reads aligned (%)

93.7

Duplicates removed (%)

10.1

Number of peaks

554 (qval < 1E-05)