GSM6402424: PDS5A-dTAG dTAG RAD21; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
RAD21
Cell type
Cell type Class
Liver
Cell type
PLC/PRF/5
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular
Attributes by original data submitter
Sample
source_name
PLC/PRF/5
cell line
PLC/PRF/5
cell type
Hepatocarcinoma cells
chip antibody
RAD21 (Abcam, ab992)
genotype
PDS5A-dTAG
treatment
dTAG
Sequenced DNA Library
library_name
GSM6402424
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, Chromatin was sheared to an average length of 300-700 bp with a Qsonica sonicator. ChIP-seq libraries were prepared using VAHTSTM Universal DNA Library Prep Kit for Illumina. After amplification and size selection with Agencourt AMPure XP beads from Beckman Coulter, the size distributions were determined with Agilent 2100 Bioanalyzer.