GSM2098806: DMSO unamp K562 3A; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous
Attributes by original data submitter
Sample
source_name
human K562 cell_DMSO_unamp
biomaterial_provider
ATCC CCL 243
cell line
K562
replicate
4
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Ten million vehicle or TOP2 poison-treated K562 cells were lysed with RIPA buffer and the lysates sonicated to fragment the DNA to 250-750bp. TOP2A was immunocaptured with anti-TOP2A IgG and Protein G magnetic beads, the bead-bound fraction separated, and immunoprecipitation repeated twice on non-bound fractions. The bead-bound fractions were combined and treated with CIP (NEB) to detach the TOP2A-bound DNA from cleavage complexes. The released DNA and input DNA from sonicated lysates were purified and used directly for library synthesis with an IlluminaTruSeq ChIP library kitor pre-amplified with a SEQXE kit (Sigma) before library preparation. Sequencing was performed on an Illumina HiSeq2500 (SE50 protocol)