LSK (Lin-Sca-1+c-kit+) cells sorted from MxCre Jak2VF/+ mouse bone marrow, 12 weeks after pI-pC injections
strain
C57BL/6
genotype/variation
MxCre Jak2VF/+
tissue
bone marrow
cell type
LSK (Lin-Sca-1+c-kit+) cell
chip antibody
H3K27me3 (Millipore, 07-449, lot 2580679)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP experiment was performed with EpiTect ChIP OneDay Kit (Qiagen) according to the manufacturer’s protocol and H3K27me3 antibody (07-449, Millipore) Libraries were prepared using Illumina TruSeq ChIP Sample Prep Kit (Illumina) with immune-precipitated DNA for each sample. The sizes of the libraries were validated utilizing the DNA 1000 chip on the Agilent 2100 Bioanalyzer. The quantification of the libraries was done using the KAPA Library Quantification Kit.