Antigen

Antigen Class

TFs and others

Antigen

CDK9

Cell type

Cell type Class

Blood

Cell type

MOLT-4

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Lymphocytic

Sample

source_name

T-ALL

cell line

MOLT4

antibody

CDK9

antibody manufacturer

Santa Cruz

antibody catalog number

C-20

drug treatment

JQ1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Standard DNA extraction from MOLT4 cell lines using cell lysis Probe sonicator 3 minutes total sonication time (1 sec on, 4 sec off, 60% amplitude) on ice library prep with Rubicon TruePlex kit

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

143109472

Reads aligned (%)

74.5

Duplicates removed (%)

12.0

Number of peaks

36055 (qval < 1E-05)

hg19

Number of total reads

143109472

Reads aligned (%)

73.7

Duplicates removed (%)

13.3

Number of peaks

33692 (qval < 1E-05)