Antigen

Antigen Class

TFs and others

Antigen

RYBP

Cell type

Cell type Class

Lung

Cell type

HBEC3-KT

Tissue

Lung, Bronchial

Cell Type

Epithelial

Disease

Normal

Sample

source_name

HBEC3-KT

cell line

HBEC3-KT

cell type

Human Bronchial cells

genotype

Wild type

chip antibody

RYBP(millipore, AB3637)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For RYBP ChIP, cells were fixed with 2.5 mM EGS (ethylene glycol-bis-succinimidyl-succinate) for 45 minutes, then fixed with 1% formaldehyde for 8 minutes; for H2AK119ub1 and H3K27me3, cells were crosslinked with 1% formaldehyde only for 8 minutes, after quenching and cell lysis, chromatin was sonicated using a Covaris E220 Evo sonicator. TruSeq ChIP Sample Preparation Kit (Illumina, IP-202-1012, IP-202-1024) was used according to the manufacturer's instructions to perform ChIP-seq library preparation. 5 ng of DNA was used as starting material for input and IP samples. Libraries were amplified using 14 cycles on the thermocycler. Libraries were validated using the Agilent High Sensitivity DNA Kit.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

47781688

Reads aligned (%)

99.0

Duplicates removed (%)

38.7

Number of peaks

1320 (qval < 1E-05)

hg19

Number of total reads

47781688

Reads aligned (%)

98.2

Duplicates removed (%)

40.0

Number of peaks

1156 (qval < 1E-05)