Antigen

Antigen Class

TFs and others

Antigen

ESR1

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MCF7

experimental conditions

2D monolayer, DMEM 2percent charcoal strip FBS

antibody

ER alpha antibody, HC-20 Santa Cruz : sc-543

Sequenced DNA Library

library_name

GSM6360821

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed for 10 min 1.5% formaldehyde. Reaction was stopped using 1.5M glycine. Cell lysates (triton and SDS based) were clarified after sonication and subjected to immunoprecipitation with ER antibody. Libarary construction was performed by : https://www.france-genomique.org/

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

119442446

Reads aligned (%)

96.3

Duplicates removed (%)

25.2

Number of peaks

16929 (qval < 1E-05)

hg19

Number of total reads

119442446

Reads aligned (%)

95.2

Duplicates removed (%)

27.4

Number of peaks

16424 (qval < 1E-05)