Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EP300

Cell type

Cell type Class
Placenta
Cell type
Trophoblast stem cells
NA
NA

Attributes by original data submitter

Sample

source_name
Placenta
tissue
Placenta
cell line
CT27
cell type
Human trophoblast stem cells
antibody
EP300

Sequenced DNA Library

library_name
GSM6341894
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed by cross-linking proteins to DNA using 1% formaldehyde solution in human TSCs. Immunoprecipitated DNA was sequenced using Illumina sequencers. Pull-downed DNA was applied for the generation of sequencing libraries using the NEB ChIP-seq library prep. kit (E7370L ) following by manufacturer's instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
24550408
Reads aligned (%)
94.9
Duplicates removed (%)
77.1
Number of peaks
8332 (qval < 1E-05)

hg19

Number of total reads
24550408
Reads aligned (%)
94.4
Duplicates removed (%)
78.0
Number of peaks
8313 (qval < 1E-05)

Base call quality data from DBCLS SRA