Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Epidermis

Cell type

Melanoma cell lines

NA

NA

Sample

source_name

EZH2_Y641F_855D_MEL_JQEZ5_H3K27_IP

strain background

C57BL/6

cell line

855

genotype/variation

EZH2 gain-of-function Y641F mutation

tissue/cell type

Melanoma tumor cell line

cell type

Melanocyte

chip antibody

H3K27me3

chip antibody vendor

Millipore

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

B cells were isolated from animals' spleen. Briefly, spleens were made into single cell suspension, stained with CD19-biotin, washed, stained with antibiotin magnetic beads and separated on an Automacs magnetic separator. Illumina Truseq ChIPseq kit

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

49152607

Reads aligned (%)

97.4

Duplicates removed (%)

7.6

Number of peaks

314 (qval < 1E-05)

mm9

Number of total reads

49152607

Reads aligned (%)

97.3

Duplicates removed (%)

7.6

Number of peaks

333 (qval < 1E-05)