Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Gonad

Cell type

Testis

MeSH Description

The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

Sample

source_name

Testis

strain

C57BL/6

tissue

testis

genotype

Phf13-/-

age

5 weeks

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was isolated using the Blueprint transcription factor protocol (blueprint-epigenome.org). Libraries were prepared using the using the TruSeq ChIP kit.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

293734953

Reads aligned (%)

27.6

Duplicates removed (%)

93.1

Number of peaks

354 (qval < 1E-05)

mm9

Number of total reads

293734953

Reads aligned (%)

27.6

Duplicates removed (%)

93.3

Number of peaks

329 (qval < 1E-05)