Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Embryo

Cell type

Trophoblasts

MeSH Description

Trophoblasts with multinuclear cytoplasm and undefined cellular structures. Syncytiotrophoblasts secrete proteolytic enzymes that erode the ENDOMETRIUM for EMBRYO IMPLANTATION. They also produce steroid, peptide, and protein hormones (PLACENTAL HORMONES).

Sample

source_name

mouse embryo

development stage

trophoblast

chip antibody

H3K4me3(Cell signaling Technology, #9727)

overexpression

B6D2F1 (C57BL/6×DBA/2)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

500cells were washed three times in 0.5% BSA-PBS solution. Lysates were clarified from micrococcal nuclease treated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to KAPA Hyper Prep Kit for illumina platform (kk8504)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

17719637

Reads aligned (%)

92.5

Duplicates removed (%)

69.2

Number of peaks

15049 (qval < 1E-05)

mm9

Number of total reads

17719637

Reads aligned (%)

92.4

Duplicates removed (%)

69.1

Number of peaks

15060 (qval < 1E-05)