Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Gonad

Cell type

Oocytes

MeSH Description

Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).

Sample

source_name

MII oocyte

development stage

MII Oocyte

chip antibody

H3K27me3(Diagnode, pAb-069-050)

overexpression

B6D2F1 (C57BL/6×DBA/2)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

500cells were washed three times in 0.5% BSA-PBS solution. Lysates were clarified from micrococcal nuclease treated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to KAPA Hyper Prep Kit for illumina platform (kk8504)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

21358513

Reads aligned (%)

99.0

Duplicates removed (%)

17.2

Number of peaks

232 (qval < 1E-05)

mm9

Number of total reads

21358513

Reads aligned (%)

98.9

Duplicates removed (%)

17.2

Number of peaks

216 (qval < 1E-05)