Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Blood

Cell type

B cells

NA

NA

Sample

source_name

B cell

genotype

Cdkn2a-/-

genotype

E{mu}-Myc

strain

C57BL/6J

chip antibody

H3K27me3 (Active Motif cat # 39155)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The ChIP-Seq sample 100 μl of the lysate (5 × 106139 cells) were used for each immunoprecipitation with anti-H3K27me3 (Active Motif cat # 39155). DNA was recovered by electroelution, phenol extraction and ethanol precipitation.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

56993064

Reads aligned (%)

99.5

Duplicates removed (%)

42.2

Number of peaks

841 (qval < 1E-05)

mm9

Number of total reads

56993064

Reads aligned (%)

99.4

Duplicates removed (%)

42.3

Number of peaks

853 (qval < 1E-05)