Antigen

Antigen Class

TFs and others

Antigen

Kat2b

Cell type

Cell type Class

Pancreas

Cell type

Islets of Langerhans

MeSH Description

Irregular microscopic structures consisting of cords of endocrine cells that are scattered throughout the PANCREAS among the exocrine acini. Each islet is surrounded by connective tissue fibers and penetrated by a network of capillaries. There are four major cell types. The most abundant beta cells (50-80%) secrete INSULIN. Alpha cells (5-20%) secrete GLUCAGON. PP cells (10-35%) secrete PANCREATIC POLYPEPTIDE. Delta cells (~5%) secrete SOMATOSTATIN.

Sample

source_name

isolated mouse islet

strain

C57BL/6

age

adult 10-12 weeks old

antibody

Kat2b (Abcam; ab12188)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP assays were performed in triplicates on approximately 600 mouse isolated islets using True MicroChIP kit (Diagenode) following manufacturer’s protocol. . ChIP-seq libraries were prepared using NEBNext-Ultra kits (New England Biolabs) following manufacturer’s instructions. DNA libraries were quantified by Qubit (Invitrogen) and were sequenced using a Hiseq 2500 instrument in single end 50pb reads (illumina).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

89260102

Reads aligned (%)

74.0

Duplicates removed (%)

90.1

Number of peaks

320 (qval < 1E-05)

mm9

Number of total reads

89260102

Reads aligned (%)

73.8

Duplicates removed (%)

90.2

Number of peaks

331 (qval < 1E-05)