Antigen

Antigen Class

TFs and others

Antigen

NFKB2

Cell type

Cell type Class

Neural

Cell type

U-87 MG

Primary Tissue

Brain

Tissue Diagnosis

Glioblastoma

Sample

source_name

U-87MG

genotype

control

cell line

U-87MG

chip antibody

p52

treatment

TWEAK

Sequenced DNA Library

library_name

GSM6329562

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a Diagenode Bioruptor. DNA was sheared to 200-500bp. ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (NEB; E7645) according to the manufacturer's protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

35221584

Reads aligned (%)

93.9

Duplicates removed (%)

40.3

Number of peaks

4827 (qval < 1E-05)

hg19

Number of total reads

35221584

Reads aligned (%)

92.9

Duplicates removed (%)

40.8

Number of peaks

4313 (qval < 1E-05)