GSM6285658: 1 % INPUT Control (transfected with scrambled siRNA) -Dex; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
NR3C1
Cell type
Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
HeLa
cell line
HeLa
cell type
Cervical cancer
chip antibody
H300 GR Santa Cruz
Sequenced DNA Library
library_name
GSM6285658
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitation (ChIP) assays were performed as previously described. Soluble chromatin was prepared using MNase digestion [50mM Tris-HCl, pH 7.5, 4mM MgCl2, 1mM CaCl2, 0.32mM sucrose, 2mM NaF, 0.2mM NaVan] with 2 units of MNase (Sigma, UK) /180μg chromatin, then immunoprecipitated with H300 GR antibody (Santa Cruz; sc-8992), RNA Polymerase II (phosphoserine-5) antibody (ACTIVE MOTIF; 39233) or rabbit non-immune serum (Santa Cruz; sc-2027). For sequencing, libraries were prepared from two replicates/condition. NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®