GSM6285567: F10-RING, rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
RNF2
Cell type
Cell type Class
Blood
Cell type
KBM-7
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous
Attributes by original data submitter
Sample
source_name
cultured cells
cell line
KBM7-1-55-S2-24 clone F10
chip antibody
MBL, #D139-3, clone 3-3
Sequenced DNA Library
library_name
GSM6285567
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Live cells were crosslinked with 1% formaldehyde for 10 min at 37C. Soluble chromatin was prepared and immunoprecipitation performed as described in Kahn et al. Nucleic Acids Res. 2016. 44:10132-10149 Libraries were prepared with NEBNext Ultra II FS DNA Library Prep Kit for Illumina (E7805) and NEBNext Multiplex Oligos for Illumina Index Primers Set 1 (E7335) according to manufacturer's instructions. Briefly, 2 ng of ChIP DNAs were fragmented for 20 minutes to the average length of 180bp, followed by adaptor ligation and 8 cycles of PCR amplification. HiSeq2500, 1x51 setup, 'HiSeq Rapid SBS Kit v2' chemistry NovaSeq SP,100bp read length, v1 sequencing chemistry