Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Blood

Cell type

MM.1S

Primary Tissue

Blood

Tissue Diagnosis

Multiple Myeloma

Sample

source_name

MM1S Multiple Myeloma cells_KDM5C70

cell line

MM1S

cell type

Multiple Myeloma cell line

treated with

KDM5-C70

chip antibody

H3K4me3

chip antibody vendor

Millpore

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed in 1% formaldehyde for 8 minutes at room temperature, sonicated on a Bioruptor Pico, incubated for an hour with pre-blocked protein A seppharose beads before being incubated with 1ug of antibody overnight at 4 degrees. The lysate was allowed to incubate with protein A beads before being washed and subejcted to proteinase K digestion Libraries were constructed using NEBnext Ultra DNA library prep kit according to manufactures instructions

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

5210676

Reads aligned (%)

99.5

Duplicates removed (%)

4.6

Number of peaks

20059 (qval < 1E-05)

hg19

Number of total reads

5210676

Reads aligned (%)

99.2

Duplicates removed (%)

4.7

Number of peaks

19960 (qval < 1E-05)