ChIP-Atlas: SRX1594763

Antigen

Cell type Class: Gonad
Cell type: Spermatogonia
MeSH Description: Euploid male germ cells of an early stage of SPERMATOGENESIS, derived from prespermatogonia. With the onset of puberty, spermatogonia at the basement membrane of the seminiferous tubule proliferate by mitotic then meiotic divisions and give rise to the haploid SPERMATOCYTES.

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: Chromatin immunoprecipitation was performed with 1x10^7 cells per experiment. The cells were collected and cross-linked for 15min in 1% paraformaldehyde, washed and lysed. Chromatin was sheared using a Bioruptor to create fragments of approximately 150 base pairs, incubated with about 2–5 μg antibody bound to 75 μl Dynabeads M-280 (Invitrogen) and rotated overnight at 4 °C, then washed and eluted. The eluted chromatin was reverse-cross-linked and column purified. ChIP samples were prepared for sequencing using Illumina TruSeq DNA Sample Preparation Kit according to the standard preparation protocol.