GSM6280750: RD-2 SMARCA4 Control 2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
SMARCA4
Cell type
Cell type Class
Muscle
Cell type
RD
Primary Tissue
Skelatal Muscle
Tissue Diagnosis
Rhabdomyosarcoma
Attributes by original data submitter
Sample
source_name
RD
cell line
RD
cell type
fusion-negative rhabdomyosarcoma
chip antibody
BRG1 (Abcam, ab110641)
genotype
control
Sequenced DNA Library
library_name
GSM6280750
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 4% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sheared on a Bioruptor Pico (Diagenode) for 20 cycles (30 sec on/ 30 sec off for each cycle) at 4°C ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Preparation Kit complemented with NEBNext Multiplex Oligos for Illumina from NEB. 10 ng of DNA was used as starting material for input samples and 1 ng for ip samples. Libraries were amplified using 15 cycles for ip samples and 12 cycles for input on the thermocycler. Post amplification libraries were size selected at 250-450bp in length using SPRISelect beads from Beckman Coulter. Libraries were validated using the Agilent D1000 ScreenTape.