Antigen

Antigen Class

TFs and others

Antigen

ESR1

Cell type

Cell type Class

Breast

Cell type

LTED cells

NA

NA

Sample

source_name

LTED breast cancer cells, Tamoxifen resistant

cell line

LTEDT

chip antibody

ERα antibody (HC-20) (Santa Cruz, catalog# sc-543)

passage

<10

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. NEB ultra kit

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

22464275

Reads aligned (%)

99.1

Duplicates removed (%)

8.9

Number of peaks

1292 (qval < 1E-05)

hg19

Number of total reads

22464275

Reads aligned (%)

98.5

Duplicates removed (%)

10.1

Number of peaks

1395 (qval < 1E-05)