Antigen

Antigen Class

TFs and others

Antigen

STAG1

Cell type

Cell type Class

Others

Cell type

Fibroblasts

NA

NA

Sample

source_name

ChIPseq_Fibroblast_SA1

antibody

SA1 (ab4457, Abcam)

treatment

NT

cell line/type

Fibroblast

Sequenced DNA Library

library_name

GSM6244243

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation was performed as previously described (Komata, M., et al., Chromatin immunoprecipitation protocol for mammalian cells. Methods Mol Biol, 2014. 1164: p. 33-8.). ChIP-seq libraries were prepared using NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (New England BioLabs).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

38484734

Reads aligned (%)

98.4

Duplicates removed (%)

35.1

Number of peaks

38126 (qval < 1E-05)

hg19

Number of total reads

38484734

Reads aligned (%)

97.9

Duplicates removed (%)

35.8

Number of peaks

38009 (qval < 1E-05)