Antigen

Antigen Class

TFs and others

Antigen

Sap130

Cell type

Cell type Class

Embryo

Cell type

Embryonic heart

NA

NA

Sample

source_name

Mouse heart

Stage

E12.5

tissue

Heart

strain

C57BL/6

chip antibody

Sap130 (A302-491A: Bethyl laboratories)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitation of Sap130 was performed with rabbit anti-Sap130 antibody (A302-491A: Bethyl laboratories, 2ug) and iDeal ChIP-seq Kit for Transcription Factors (Diagenode) following the manufacturer's protocol ChIP-seq libraries were generated using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB) following the manufacturer's protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

56251060

Reads aligned (%)

99.2

Duplicates removed (%)

41.8

Number of peaks

15167 (qval < 1E-05)

mm9

Number of total reads

56251060

Reads aligned (%)

99.1

Duplicates removed (%)

41.8

Number of peaks

15164 (qval < 1E-05)