Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

B cells

NA

NA

Sample

source_name

Act B cell

strain

C57BL/6

genotype

Cd23-Cre Tcf3,4(fl/fl)

cell preparation

2-day anti-CD40 rIL-4 stimulation whole population

cell sorting strategy

whole population

chip antibody

H3K27ac (abcam)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

phenol-chloroform extraction

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

30033876

Reads aligned (%)

98.6

Duplicates removed (%)

8.5

Number of peaks

24833 (qval < 1E-05)

mm9

Number of total reads

30033876

Reads aligned (%)

98.5

Duplicates removed (%)

8.6

Number of peaks

24837 (qval < 1E-05)