Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Uterus

Cell type

HeLa

Primary Tissue

Cervix

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

uterine cervix

cell line

HeLa cell

tissue

uterine cervix

chip antibody

polII-Ser5(abcam,ab5131)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated whole cells and Polymerrase II were isolated with antibody. Libraries were generated with TruSeq ChIP-seq Sample Prep Kit (Illumina) according to the manufacturer’s instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

53669366

Reads aligned (%)

52.2

Duplicates removed (%)

2.8

Number of peaks

816 (qval < 1E-05)

hg19

Number of total reads

53669366

Reads aligned (%)

51.6

Duplicates removed (%)

3.0

Number of peaks

324 (qval < 1E-05)