Curated Sample Data


Genome
dm3
Antigen Class
Histone
Antigen
H3K27me3
Cell type Class
Larvae
Cell type
CNS and imaginal discs

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
3rd instar brains and imaginal discs
genotype/variation
cgA22 mutant
developmental stage
3rd instar larva
tissue
CNS and imaginal discs
chip antibody
anti-H3K27me3 (Millipore, 17-622)

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Fixed brains and imaginal discs were dissected from 10 third instar larvae, and sonicated chromatin incubated with anti ChIP was performed with 1:100 dilutions of anti-Cg [custom made], anti-Ph (a kind gift from Donna Arndt-Jovin) antibodies and 1:200 dilutions of anti-H3K27me3 (Millipore, 17-622) antibodies. Input ChIP DNA (30 ng) is blunt-ended and phosphorylated. A single 'A' nucleotide is added to the 3' ends of the fragments in preparation for ligation to an adapter that has a single-base 'T' overhang. The ligation products are purified and accurately size-selected by agarose gel electrophoresis. Size-selected DNA is purified and PCR-amplified to enrich for fragments that have adapters on both ends. The final purified product is then quantitated before cluster generation. http://ethanomics.wordpress.com/chip-seq-library-construction-using-the-illumina-truseq-adapters/.

Platform Information


instrument_model
Illumina HiSeq 2500

External Database Query

Logs in read processing pipeline


Number of total reads
4556468
Reads aligned (%)
26.7
Duplicates removed (%)
28.6
Number of peaks
2513 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA