Antigen

Antigen Class

Histone

Antigen

H3K64ac

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

46c_H3K64ac

cell line

46c (sox1-GFP)

cell type

mouse embryonic stem cells

passages

25-28

chip antibody

anti-H3K64ac (Di Cerbo et al 2014, PMID: 24668167)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was digested with MNase and released at 4C overnight, then histone-DNA complexes were isolated with antibody DNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

201973997

Reads aligned (%)

90.4

Duplicates removed (%)

60.7

Number of peaks

14691 (qval < 1E-05)

mm9

Number of total reads

201973997

Reads aligned (%)

90.3

Duplicates removed (%)

60.8

Number of peaks

14677 (qval < 1E-05)