Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Bone

Cell type

U2OS

Tissue

bone

Lineage

mesoderm

Description

osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Sample

source_name

U2OS cells

antibody

non

cell line

U2OS

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, libraries from GEO record GSE44672 were used and sequenced deeper.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

hg38

Number of total reads

16948398

Reads aligned (%)

99.1

Duplicates removed (%)

2.7

Number of peaks

544 (qval < 1E-05)

hg19

Number of total reads

16948398

Reads aligned (%)

98.2

Duplicates removed (%)

4.3

Number of peaks

895 (qval < 1E-05)