Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Blood

Cell type

Th1 Cells

MeSH Description

A subset of helper-inducer T-lymphocytes which synthesize and secrete INTERLEUKIN-2; INTERFERON-GAMMA; and INTERLEUKIN-12. Due to their ability to kill antigen-presenting cells and their lymphokine-mediated effector activity, Th1 cells are associated with vigorous delayed-type hypersensitivity reactions.

Sample

source_name

Th1

strain

C57BL/6

cell type

Th1

dna fragmentation method

MNase to generate approximately 80% mononucleosomes and 20% dinucleosomes

chip antibody

H3K27me3 (07-449, Upstate)

genotype/variation

WT

Sequenced DNA Library

library_name

GSM949924: WT H3K27me3 ChIP-seq

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

29858408

Reads aligned (%)

89.1

Duplicates removed (%)

40.0

Number of peaks

127 (qval < 1E-05)

mm9

Number of total reads

29858408

Reads aligned (%)

89.0

Duplicates removed (%)

40.0

Number of peaks

117 (qval < 1E-05)