Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
pho

Cell type

Cell type Class
Larvae
Cell type
Imaginal brain disc
NA
NA

Attributes by original data submitter

Sample

source_name
3rd instar brains and imaginal disks
genotype
ry- enPREs deleted
tissue
CNS and imaginal disks
developmental stage
3rd instar
chip antibody
anti-Pho

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Fixed brains and imaginal discs were dissected from 10 third instar larvae, and sonicated chromatin incubated with anti ChIP was performed with 1:100 dilutions of anti-Pho [custom made], anti-Ph (a kind gift from Donna Arndt-Jovin) antibodies and 1:200 dilutions of anti-H3K27me3 (Millipore, 17-622) antibodies.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

dm3

Number of total reads
1526261
Reads aligned (%)
28.9
Duplicates removed (%)
19.5
Number of peaks
795 (qval < 1E-05)

dm6

Number of total reads
1526261
Reads aligned (%)
28.2
Duplicates removed (%)
20.8
Number of peaks
838 (qval < 1E-05)

Base call quality data from DBCLS SRA