GSM2046003: Sarcoma MEF Dox ON 12h; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast
Attributes by original data submitter
Sample
source_name
Sarcoma iPSCs #3-derived MEF
cell type
sarcoma MEF
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. We used anti HA antibody(Monoclonal Mouse IgG 1-κ):nacalai cat# 06340-54 Lot#M4H0340 . Libraries were prepared according to illumina'sTrueseq ChIP sample prep kit set A(Part#IP-202-1012)