GSM6182614: T47D, FOXA1, biol rep2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
FOXA1
Cell type
Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal
Attributes by original data submitter
Sample
source_name
T-47D
disease state
breast cancer
cell line
T-47D
cell type
Human breast epithelial
chip antibody
FOXA1 (Diagenode(#C15410231-100)
Sequenced DNA Library
library_name
GSM6182614
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed and immunopreciptated using Diagenode iDeal ChIP-seq kit for Transcription Factors per the manufacturers' instructions. For TLE3 immunopreciptations, cells were crosslinked using Diagenode ChIP Cross-link Gold prior to formaldehyde fixation. Library preparation was performed by Diagenode. Briefly, libraries were prepared using IP-Star Compact Automated System from both INPUT and ChIP DNA samples (starting amount of 3µg for IP and 1.5µg for INPUT) with the MIcroPlex Library Preparation Kit v2 (Diagenode #C05010013). Library amplification was assessed by qPCR, purified, and quantified with a Qubit dsDNA HS Assay Kit (Thermo Fisher Q32854). Fragment size was analyzed using High Sensitivity NGS Fragment Analysis Kit on an Agilent Fragment Analyzer.