Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Mouse embryonic stem cells
strain
129SvEv
cell type
Mouse embryonic stem cells
passage
15-20
genotype/variation
Arid1aflfl
treated with
ethanol (EtOH)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were obtained from sonicated nuclei crosslinked with formaldehyde, and protein-DNA complexes were isolated by immunoprecipitation. All libraries were barcoded and prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
28736363
Reads aligned (%)
98.0
Duplicates removed (%)
8.9
Number of peaks
511 (qval < 1E-05)

mm9

Number of total reads
28736363
Reads aligned (%)
97.8
Duplicates removed (%)
9.0
Number of peaks
564 (qval < 1E-05)

Base call quality data from DBCLS SRA