Antigen

Antigen Class

TFs and others

Antigen

Suz12

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

Mouse embryonic stem cells

strain

129SvEv

cell type

Mouse embryonic stem cells

passage

15-20

genotype/variation

Smarca4flfl

treated with

0.8 uM 4-hydroxytamoxifen (Tam)

chip antibody

Rabbit anti-Suz12

chip antibody vendor

Active Motif

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were obtained from sonicated nuclei crosslinked with formaldehyde, and protein-DNA complexes were isolated by immunoprecipitation. All libraries were barcoded and prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

21331962

Reads aligned (%)

97.7

Duplicates removed (%)

10.6

Number of peaks

1034 (qval < 1E-05)

mm9

Number of total reads

21331962

Reads aligned (%)

97.4

Duplicates removed (%)

10.7

Number of peaks

513 (qval < 1E-05)