Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

Mouse embryonic stem cells

strain

129SvEv

cell type

Mouse embryonic stem cells

passage

15-20

genotype/variation

expressing WT Smarca4V5

treated with

none (untreated)

chip antibody

Rabbit anti-H3K27me3

chip antibody vendor

EMD Millipore

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were obtained from sonicated nuclei crosslinked with formaldehyde, and protein-DNA complexes were isolated by immunoprecipitation. All libraries were barcoded and prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

34581836

Reads aligned (%)

97.8

Duplicates removed (%)

9.1

Number of peaks

598 (qval < 1E-05)

mm9

Number of total reads

34581836

Reads aligned (%)

97.6

Duplicates removed (%)

9.1

Number of peaks

605 (qval < 1E-05)