Antigen

Antigen Class

Histone

Antigen

H3K36me3

Cell type

Cell type Class

Digestive tract

Cell type

HT-29

Primary Tissue

Colon

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

H3K36me3_DMSO

cell line

HT29

cell type

colorectal adenocarcinoma

agent

DMSO

time

ctrl

chip antibody

anti-H3K36me3 (Abcam, ab9050)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq was performed as previously described (Reddy et al. Molecular Cell 2013). RNA-seq was performed as previously outlned (Gertz et al. 2012) Illumina Single End for ChIP-seq, Illumina Paired End for RNA-seq

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

64431642

Reads aligned (%)

98.0

Duplicates removed (%)

6.7

Number of peaks

18971 (qval < 1E-05)

hg19

Number of total reads

64431642

Reads aligned (%)

97.8

Duplicates removed (%)

7.3

Number of peaks

19021 (qval < 1E-05)