Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Embryo
Cell type
Epiblast
NA
NA

Attributes by original data submitter

Sample

source_name
E6.5 epiblast
strain
C57BL/6N x PWK
tissue/cell type
E6.5 epiblast
chip antibody
H3K27Me3
chip antibody vendor
Diagenode

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
embryonic cells were washed with PBS and incubated in lysis buffer. ChIP-Seq libraries were prepared using TELP developed by PengXu et al. (PMID: 25223787) with slightly modification.

Sequencing Platform

instrument_model
Illumina HiSeq 1500

mm10

Number of total reads
102258162
Reads aligned (%)
17.2
Duplicates removed (%)
47.0
Number of peaks
182 (qval < 1E-05)

mm9

Number of total reads
102258162
Reads aligned (%)
17.1
Duplicates removed (%)
47.0
Number of peaks
159 (qval < 1E-05)

Base call quality data from DBCLS SRA