SRX1533821
GSM2041072: ChIP-Seq PN5 zygote K27Me3 rep1; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Embryo
Cell type
Preimplantation embryo
NA
NA

Attributes by original data submitter

Sample

source_name
Preimplantation embryo
strain
C57BL/6N x PWK
tissue/cell type
Preimplantation embryo
chip antibody
H3K27Me3
chip antibody vendor
Diagenode

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
After removing the zona pellucida, embryonic cells were washed with PBS and incubated in lysis buffer. ChIP-Seq libraries were prepared using TELP developed by PengXu et al. (PMID: 25223787) with slightly modification.

Sequencing Platform

instrument_model
Illumina HiSeq 1500

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
59381416
Reads aligned (%)
59.0
Duplicates removed (%)
27.2
Number of peaks
376 (qval < 1E-05)

mm9

Number of total reads
59381416
Reads aligned (%)
58.9
Duplicates removed (%)
27.3
Number of peaks
368 (qval < 1E-05)

Base call quality data from DBCLS SRA